Abstract：High levels of reactive oxygen species (ROS), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to    cryopreservation extenders influenced post-thaw sperm characteristics and fertility in Acipenser dabry anus,Acipenser sinensis and Acipenser baerii. Prior to freezing,sperm samples were diluted with a base extender as control or in extender supplemented with catalase (CAT), glutathione (GSH), cysteine (NAC), ascorbic acid (VC) or their paired combinations. Protective concentr-ations of CAT, GSH and VC in the three species were 25 U/ml, 0.25-0.5 mg/ml and 0.5 mg/ml, respectively. Cysteine showed no protective effect against ROS. The addition of CAT,GSH and VC positively affected either acrosome or membrane integrity of post-thawed sperm in the three species, as well as spermato zoan motility in A. sinensis. The combination of antioxidants    did not show a positive synergistic effect. This study suggested that the use of antioxidants in the cryopreser vation of sturgeon sperm has potential to decrease intracellular ROS, and consequently preserve acrosome and membrane integrity, as well  as     spermatozoan motility.

Li P., Xi M.D., Du H., Qiao X.M., Liu Z.G., Wei Q.W.*.Reproduction in Domestic Animals. 2017. DOI: 10.1111/rda.13103.

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