Abstract：Hucho hucho, one of the most endangered members of the family Salmonidae, is endemic to the Danube basin. In this study, the complete mitochondrial genome of H. hucho was sequenced and characterized. The genome is 16,751 bp in length and contains 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and a noncoding control region. The gene arrangement and nucleotide composition of the mitochondrial genome are similar to those of H. bleekeri. An 82 bp tandem repeat was identified in the control region, which is variable in length and copy number of repeat between and within species. The complete mitochondrial DNA of H. hucho should be useful to study population genetics, biogeography and adaptive evolution of this lineage.